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ATCC human ovarian cancer cell lines
Human Ovarian Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ovarian cancer cell lines/product/ATCC
Average 97 stars, based on 1248 article reviews

human ovarian cancer cell lines - by Bioz Stars, 2026-03

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Effects of lncRNA-PRLB knockdown on ovarian cell progression and drug resistance. CAOV3 and SKOV3 cells were transfected with scrambled siRNA (siNC) or lncRNA-PRLB siRNA; 48 h after transfection, (A,B) lncRNA-PRLB expression was determined using qRT-PCR; (C,D) cell proliferation was evaluated using the EdU incorporation assay; (E,F) cell viability was determined using the CCK-8 assay; (G,H) cell apoptosis was measured using the TUNEL assay; (I,J) caspase-3 activity was determined using the caspase-3 activity assay; (K,L) cell invasion was assessed using the Transwell invasion assay; and (M,N) cell migration was determined using the wound healing assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Medicine

Article Title: LncRNA-PRLB drives ovarian cancer progression and chemoresistance by stabilizing GPX4 mRNA through the FUS-mediated suppression of ferroptosis

doi: 10.3389/fmed.2026.1759058

Figure Lengend Snippet: Effects of lncRNA-PRLB knockdown on ovarian cell progression and drug resistance. CAOV3 and SKOV3 cells were transfected with scrambled siRNA (siNC) or lncRNA-PRLB siRNA; 48 h after transfection, (A,B) lncRNA-PRLB expression was determined using qRT-PCR; (C,D) cell proliferation was evaluated using the EdU incorporation assay; (E,F) cell viability was determined using the CCK-8 assay; (G,H) cell apoptosis was measured using the TUNEL assay; (I,J) caspase-3 activity was determined using the caspase-3 activity assay; (K,L) cell invasion was assessed using the Transwell invasion assay; and (M,N) cell migration was determined using the wound healing assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Human ovarian cancer cell lines CAOV3 and SKOV3 were obtained from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Knockdown, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, TUNEL Assay, Activity Assay, Caspase-3 Activity Assay, Transwell Invasion Assay, Migration, Wound Healing Assay

Effects of lncRNA-PRLB overexpression on ovarian cell progression and drug resistance. CAOV3 and SKOV3 cells were transfected with empty plasmid (Ctrl group) or lncRNA-PRLB-overexpressing vector (OE group); 48 h after transfection, (A,B) lncRNA-PRLB expression was determined using qRT-PCR; (C,D) cell proliferation was evaluated using the EdU incorporation assay; (E,F) cell viability was determined using the CCK-8 assay; (G,H) caspase-3 activity was determined using the caspase-3 activity assay; (I,J) cell invasion was assessed using the Transwell invasion assay; and (K,L) cell migration was determined using the wound healing assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Medicine

Article Title: LncRNA-PRLB drives ovarian cancer progression and chemoresistance by stabilizing GPX4 mRNA through the FUS-mediated suppression of ferroptosis

doi: 10.3389/fmed.2026.1759058

Figure Lengend Snippet: Effects of lncRNA-PRLB overexpression on ovarian cell progression and drug resistance. CAOV3 and SKOV3 cells were transfected with empty plasmid (Ctrl group) or lncRNA-PRLB-overexpressing vector (OE group); 48 h after transfection, (A,B) lncRNA-PRLB expression was determined using qRT-PCR; (C,D) cell proliferation was evaluated using the EdU incorporation assay; (E,F) cell viability was determined using the CCK-8 assay; (G,H) caspase-3 activity was determined using the caspase-3 activity assay; (I,J) cell invasion was assessed using the Transwell invasion assay; and (K,L) cell migration was determined using the wound healing assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Human ovarian cancer cell lines CAOV3 and SKOV3 were obtained from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, CCK-8 Assay, Activity Assay, Caspase-3 Activity Assay, Transwell Invasion Assay, Migration, Wound Healing Assay

Effects of lncRNA-PRLB knockdown on the ferroptosis of ovarian cancer cells. CAOV3 and SKOV3 cells were transfected with scrambled siRNA (siNC) or lncRNA-PRLB siRNA; 48 h after transfection, (A,B) ROS levels were determined using the ROS fluorescence detection kit; (C,D) MDA levels were measured using the MDA kit; (E,F) Fe2 + levels in cells were measured using the Fe2 + content assay kit; (G,H) LDH levels were measured using the LDH release assay; (I,J) GSH levels were determined using the GSH assay kit; (K,L) GPX4 mRNA expression was determined using qRT-PCR; and (M,N) GPX4 protein levels were determined using the Western blot assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Medicine

Article Title: LncRNA-PRLB drives ovarian cancer progression and chemoresistance by stabilizing GPX4 mRNA through the FUS-mediated suppression of ferroptosis

doi: 10.3389/fmed.2026.1759058

Figure Lengend Snippet: Effects of lncRNA-PRLB knockdown on the ferroptosis of ovarian cancer cells. CAOV3 and SKOV3 cells were transfected with scrambled siRNA (siNC) or lncRNA-PRLB siRNA; 48 h after transfection, (A,B) ROS levels were determined using the ROS fluorescence detection kit; (C,D) MDA levels were measured using the MDA kit; (E,F) Fe2 + levels in cells were measured using the Fe2 + content assay kit; (G,H) LDH levels were measured using the LDH release assay; (I,J) GSH levels were determined using the GSH assay kit; (K,L) GPX4 mRNA expression was determined using qRT-PCR; and (M,N) GPX4 protein levels were determined using the Western blot assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Human ovarian cancer cell lines CAOV3 and SKOV3 were obtained from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Knockdown, Transfection, Fluorescence, Lactate Dehydrogenase Assay, GSH Assay, Expressing, Quantitative RT-PCR, Western Blot

GPX4 overexpression and ferrostatin-1 attenuated the effects of lncRNA-PRLB knockdown on ovarian cancer cell progression and paclitaxel resistance. CAOV3 and SKOV3 cells were transfected with an empty plasmid (Ctrl group) or a GPX4-overexpressing vector (OE_GPX4 group); 48 h after transfection, (A,B) GPX4 mRNA expression was determined using qRT-PCR; (C,D) GPX4 protein expression was determined using the western blot assay. (E–P) CAOV3 and SKOV3 cells were co-transfected with si-lncRNA-PRLB and OE_GPX4 or co-treated with ferrostatin-1 followed by si-lncRNA-PRLB transfection for 48 h; (E,F) cell proliferation was evaluated using the EdU incorporation assay; (G,H) cell viability was determined using the CCK-8 assay; (I,J) cell apoptosis was measured using the TUNEL assay; (K,L) caspase-3 activity was determined using the caspase-3 activity assay; (M,N) cell invasion was assessed using the transwell invasion assay; and (O,P) cell migration was determined using the wound healing assay. (Q,R) CAOV3/Tax and SKOV3/Tax cells were co-transfected with si-lncRNA-PRLB and OE_GPX4 or co-treated with ferrostatin-1 followed by si-lncRNA-PRLB transfection for 48 h; the IC50 values of paclitaxel were determined using the CCK-8 assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Medicine

Article Title: LncRNA-PRLB drives ovarian cancer progression and chemoresistance by stabilizing GPX4 mRNA through the FUS-mediated suppression of ferroptosis

doi: 10.3389/fmed.2026.1759058

Figure Lengend Snippet: GPX4 overexpression and ferrostatin-1 attenuated the effects of lncRNA-PRLB knockdown on ovarian cancer cell progression and paclitaxel resistance. CAOV3 and SKOV3 cells were transfected with an empty plasmid (Ctrl group) or a GPX4-overexpressing vector (OE_GPX4 group); 48 h after transfection, (A,B) GPX4 mRNA expression was determined using qRT-PCR; (C,D) GPX4 protein expression was determined using the western blot assay. (E–P) CAOV3 and SKOV3 cells were co-transfected with si-lncRNA-PRLB and OE_GPX4 or co-treated with ferrostatin-1 followed by si-lncRNA-PRLB transfection for 48 h; (E,F) cell proliferation was evaluated using the EdU incorporation assay; (G,H) cell viability was determined using the CCK-8 assay; (I,J) cell apoptosis was measured using the TUNEL assay; (K,L) caspase-3 activity was determined using the caspase-3 activity assay; (M,N) cell invasion was assessed using the transwell invasion assay; and (O,P) cell migration was determined using the wound healing assay. (Q,R) CAOV3/Tax and SKOV3/Tax cells were co-transfected with si-lncRNA-PRLB and OE_GPX4 or co-treated with ferrostatin-1 followed by si-lncRNA-PRLB transfection for 48 h; the IC50 values of paclitaxel were determined using the CCK-8 assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Human ovarian cancer cell lines CAOV3 and SKOV3 were obtained from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Over Expression, Knockdown, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, TUNEL Assay, Activity Assay, Caspase-3 Activity Assay, Transwell Invasion Assay, Migration, Wound Healing Assay

GPX4 overexpression and ferrostatin-1 attenuated the effects of lncRNA-PRLB knockdown on the ferroptosis of ovarian cancer cells. CAOV3 and SKOV3 cells were transfected with empty plasmid (Ctrl group) or GPX4-overexpressing vector (OE_GPX4 group); 48 h after transfection, (A,B) ROS levels were determined using the ROS fluorescence detection kit; (C,D) MDA levels were measured using the MDA kit; (E,F) Fe 2+ levels in cells were measured using the Fe 2+ content assay kit; (G,H) LDH levels were measured using the LDH release assay; and (I,J) GSH levels were determined using the GSH assay kit. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Medicine

Article Title: LncRNA-PRLB drives ovarian cancer progression and chemoresistance by stabilizing GPX4 mRNA through the FUS-mediated suppression of ferroptosis

doi: 10.3389/fmed.2026.1759058

Figure Lengend Snippet: GPX4 overexpression and ferrostatin-1 attenuated the effects of lncRNA-PRLB knockdown on the ferroptosis of ovarian cancer cells. CAOV3 and SKOV3 cells were transfected with empty plasmid (Ctrl group) or GPX4-overexpressing vector (OE_GPX4 group); 48 h after transfection, (A,B) ROS levels were determined using the ROS fluorescence detection kit; (C,D) MDA levels were measured using the MDA kit; (E,F) Fe 2+ levels in cells were measured using the Fe 2+ content assay kit; (G,H) LDH levels were measured using the LDH release assay; and (I,J) GSH levels were determined using the GSH assay kit. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Human ovarian cancer cell lines CAOV3 and SKOV3 were obtained from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Over Expression, Knockdown, Transfection, Plasmid Preparation, Fluorescence, Lactate Dehydrogenase Assay, GSH Assay

Stability of GPX4 mRNA regulated by lncRNA-PRLB and FUS1. (A) The FUS was pulled down by lncRNA-PLBR in CAOV3 cells. (B) The FUS was pulled down by GPX4 mRNA in CAOV3 cells. (C) The relative enrichment of lncRNA-PRLB by FUS in CAOV3 cells. (D) The relative enrichment of GPX4 by FUS in CAOV3 cells. (E) The relative expression of GPX4 at different time points of 10 μg/mL actinomycin D treatment after lncRNA-PRLB knockdown in COAV3 cells. (F) The relative expression of GPX4 at different time points of 10 μg/mL actinomycin D treatment after lncRNA-PRLB overexpression in COAV3 cells. (G) The relative expression of FUS mRNA after FUS siRNA transfection in CAOV3 cells. (H) The relative expression of GPX4 at different time points of 10 μg/mL actinomycin D treatment after FUS knockdown in COAV3 cells. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Medicine

Article Title: LncRNA-PRLB drives ovarian cancer progression and chemoresistance by stabilizing GPX4 mRNA through the FUS-mediated suppression of ferroptosis

doi: 10.3389/fmed.2026.1759058

Figure Lengend Snippet: Stability of GPX4 mRNA regulated by lncRNA-PRLB and FUS1. (A) The FUS was pulled down by lncRNA-PLBR in CAOV3 cells. (B) The FUS was pulled down by GPX4 mRNA in CAOV3 cells. (C) The relative enrichment of lncRNA-PRLB by FUS in CAOV3 cells. (D) The relative enrichment of GPX4 by FUS in CAOV3 cells. (E) The relative expression of GPX4 at different time points of 10 μg/mL actinomycin D treatment after lncRNA-PRLB knockdown in COAV3 cells. (F) The relative expression of GPX4 at different time points of 10 μg/mL actinomycin D treatment after lncRNA-PRLB overexpression in COAV3 cells. (G) The relative expression of FUS mRNA after FUS siRNA transfection in CAOV3 cells. (H) The relative expression of GPX4 at different time points of 10 μg/mL actinomycin D treatment after FUS knockdown in COAV3 cells. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Human ovarian cancer cell lines CAOV3 and SKOV3 were obtained from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Expressing, Knockdown, Over Expression, Transfection

FUS knockdown attenuated the effects of lncRNA-PRLB knockdown on ovarian cancer cell progression, ferroptosis, and paclitaxel resistance. CAOV3 cells were co-transfected with lncRNA-PRLB-overexpressing plasmid and si-FUS for 48 h; (A) Cell proliferation was evaluated using the EdU incorporation assay; (B) Cell viability was determined using the CCK-8 assay; (C) Caspase-3 activity was determined using the caspase-3 activity assay; (D) Cell invasion was assessed using the transwell invasion assay; (E) Cell migration was determined using the wound healing assay; (F) ROS levels were determined using the ROS fluorescence detection kit; (G) MDA levels were measured using the MDA kit; (H) Fe 2+ levels in cells were measured using the Fe 2+ content assay kit; (I) LDH levels were measured using the LDH release assay; (J) GSH levels were determined using the GSH assay; and (K) IC50 values of paclitaxel were determined using the CCK-8 assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Medicine

Article Title: LncRNA-PRLB drives ovarian cancer progression and chemoresistance by stabilizing GPX4 mRNA through the FUS-mediated suppression of ferroptosis

doi: 10.3389/fmed.2026.1759058

Figure Lengend Snippet: FUS knockdown attenuated the effects of lncRNA-PRLB knockdown on ovarian cancer cell progression, ferroptosis, and paclitaxel resistance. CAOV3 cells were co-transfected with lncRNA-PRLB-overexpressing plasmid and si-FUS for 48 h; (A) Cell proliferation was evaluated using the EdU incorporation assay; (B) Cell viability was determined using the CCK-8 assay; (C) Caspase-3 activity was determined using the caspase-3 activity assay; (D) Cell invasion was assessed using the transwell invasion assay; (E) Cell migration was determined using the wound healing assay; (F) ROS levels were determined using the ROS fluorescence detection kit; (G) MDA levels were measured using the MDA kit; (H) Fe 2+ levels in cells were measured using the Fe 2+ content assay kit; (I) LDH levels were measured using the LDH release assay; (J) GSH levels were determined using the GSH assay; and (K) IC50 values of paclitaxel were determined using the CCK-8 assay. N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Human ovarian cancer cell lines CAOV3 and SKOV3 were obtained from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Knockdown, Transfection, Plasmid Preparation, CCK-8 Assay, Activity Assay, Caspase-3 Activity Assay, Transwell Invasion Assay, Migration, Wound Healing Assay, Fluorescence, Lactate Dehydrogenase Assay, GSH Assay

A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.

Journal: bioRxiv

Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

doi: 10.64898/2026.01.28.702332

Figure Lengend Snippet: A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.

Article Snippet: Human ovarian cancer cell lines SKOV3 (ATCC, HTB-77), CAOV3 (ATCC, HTB-75), and CAOV4 (ATCC, HTB-76) were obtained from the American Type Culture Collection.

Techniques: Derivative Assay, Expressing, Transplantation Assay, Microscopy, Isolation

A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.

Journal: bioRxiv

Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

doi: 10.64898/2026.01.28.702332

Figure Lengend Snippet: A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.

Article Snippet: Human ovarian cancer cell lines SKOV3 (ATCC, HTB-77), CAOV3 (ATCC, HTB-75), and CAOV4 (ATCC, HTB-76) were obtained from the American Type Culture Collection.

Techniques: Expressing, RNA Sequencing, MANN-WHITNEY, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Transduction, shRNA

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